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recombinant human egf  (R&D Systems)


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    R&D Systems recombinant human egf
    Recombinant Human Egf, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+recombinant+egf/Recombinant+Human+EGF+Protein%2C+CF/pmc13091051-268-9-12
    Average 97 stars, based on 1262 article reviews
    recombinant human egf - by Bioz Stars, 2026-09
    97/100 stars

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    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD.
    Article Snippet: The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%). .. Human enteroid 2D monolayer differentiation The collagen IV stock solution was prepared by dissolving collagen IV (1 mg ml−1; Sigma-Aldrich, C5533-5MG) in 100 mM acetic acid (0.6% acetic acid in H2O).

    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD
    Article Snippet: The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%). .. The collagen IV stock solution was prepared by dissolving collagen IV (1 mg ml −1 ; Sigma-Aldrich, C5533-5MG) in 100 mM acetic acid (0.6% acetic acid in H 2 O).

    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD
    Article Snippet: .. The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%).

    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD.
    Article Snippet: .. The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%).

    Recombinant:

    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD.
    Article Snippet: The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%). .. Human enteroid 2D monolayer differentiation The collagen IV stock solution was prepared by dissolving collagen IV (1 mg ml−1; Sigma-Aldrich, C5533-5MG) in 100 mM acetic acid (0.6% acetic acid in H2O).

    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD
    Article Snippet: The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%). .. The collagen IV stock solution was prepared by dissolving collagen IV (1 mg ml −1 ; Sigma-Aldrich, C5533-5MG) in 100 mM acetic acid (0.6% acetic acid in H 2 O).

    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD
    Article Snippet: .. The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l -glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml −1 , human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml −1 , Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N -acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E 2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%).

    Article Title: ECM1 expression in chronic liver disease: Regulation by EGF/STAT1 and IFNγ/NRF2 signalling
    Article Snippet: Reagents Mouse recombinant EGF (354001), HGF (2207-HG-025) and IFNγ (315-05) were purchased from Corning, R&D and PeproTech, respectively. .. Human recombinant EGF (AF-100-15) and HGF (294-HG-005) were from PeproTech and R&D systems. .. Erlotinib (5083S), Acetylcysteine (HY-B0215), Oltipraz (sc-205777), hydrogen peroxide (1072102500) were from Cell signaling, MedChemExpress, Santa Cruz, and Sigma, respectively.

    Article Title: Fructose and glucose from sugary drinks enhance colorectal cancer metastasis via SORD.
    Article Snippet: .. The medium for culturing human colon tumour organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 3 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1.25 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, Noggin (10% conditioned medium), R-spondin (10% conditioned medium) and penicillin–streptomycin (Gibco, 15140-122) at 1× concentration. .. The medium for culturing human colon organoids contained the following components: Advanced DMEM/F12 (Gibco, 12634010), B27 Supplement (Invitrogen, 17504001) at 1× concentration, l-glutamine (Gibco, 25030164) at 2 mM, HEPES (Amresco, J848) at 10 mM, Primocin (Invitrogen, NC9141851) at 100 μg ml−1, human recombinant EGF (R&D Systems, 236-EG-200) at 50 ng ml−1, Gastrin I (Sigma-Aldrich, SCP0152) at 10 nM, Y-27632 (cAMP inhibitor; Selleck Chemicals, S1049) at 10 μM, A-83-01 (TGF-β inhibitor; R&D Systems, 2939) at 500 nM, SB202190 (MAPK inhibitor; Selleck Chemicals, S1077) at 10 μM, nicotinamide (Sigma-Aldrich, N3376) at 10 mM, N-acetylcysteine (Sigma-Aldrich, A9165) at 1 mM, prostaglandin E2 (Sigma-Aldrich, P0409) at 10 nM, penicillin–streptomycin (Gibco, 15140-122) at 1× concentration, Noggin conditioned medium (10%), R-spondin conditioned medium (10%) and WNT-3A conditioned medium (30%).

    Article Title: The histone modifier KAT2A presents a selective target in a subset of well-differentiated microsatellite-stable colorectal cancers.
    Article Snippet: Singularized cells were cultured in ultra-low attachment flasks (Corning, NY, USA) in serum-free culture medium (Advanced DMEM/F-12 supplemented with 0.6% glucose, 2 mM L-glutamine (ThermoFisher Scientific, Waltham, MA, USA), 5 mM HEPES, 4 μg/mL heparin (Sigma-Aldrich, St. Louis, MO, USA), 4 mg/mL bovine serum albumin (PAN-Biotech, Aidenbach, Germany)). .. Human recombinant EGF (20 ng/mL) and human recombinant FGF (10 ng/ mL) (R&D Systems, Minneapolis, MN, USA) were added twice per week. .. CRISPR-interference-based KAT2A knockdown dCas9-KRAB-expressing CRC cells were generated by lentiviral transduction with pLV hUbC-dCas9 KRAB-T2A-GFP, a kind gift from Charles Gersbach (Addgene #67620) [17].

    Article Title: Shaped organoid compositions and methods of making same
    Article Snippet: .. Materials: mTeSR1 media (StemCell Technologies); Advanced DMEM-F12 (Invitrogen); RPMI 1640 (Invitrogen); hESC qualified Matrigel (Corning); Defined FBS (Hyclone); L-glutamine (100×) (Invitrogen); penicillin-streptomycin (100×) (Invitrogen); 50×B27 supplement (Invitrogen); HEPES buffer (Invitrogen); Dispase (Invitrogen); Activin A (R&D Systems); FGF4 (R&D Systems); CHIR99021 (R&D Systems); polydimethylsiloxane (PDMS) tissue culture collection channel scaffold with appropriately sized grooves; GFR Matrigel, phenol red-free (Corning); Minigut media: Advanced DMEM-F12 medium supplemented with 2 mM glutamine, 10 mM HEPES, 100 U/mL penicillin, 100 μg/mL streptomycin, 1×N2 supplement, 1×B27 supplement; human recombinant EGF (R&D Systems); Dumont #4 forceps (Fine Science Tools); Tissue Train Culture Plates with nylon mesh anchors (FlexCell International). ..



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